By Debra J. Shetlar, Patricia Chevez-Barrios, Sander Dubovy, Robert H. Rosa, Jr, Nasreen Syed, Matthew W. Wilson, Ron W. Pelton, Jacob Pe'er
Discusses advances within the analysis and class of tumors because it courses the reader via a logical, tissue-specific series that levels from topography via affliction approach to normal and differential prognosis. Many new colour pathologic and medical photos and diagrams spotlight this newest revision. Covers wound fix; specimen dealing with, together with processing and marking; and diagnostic innovations. additionally contains a record for inquiring for ophthalmic pathologic session.
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Extra info for Basic and Clinical Science Course, 2008-2009, Section 4: Ophthalmic Pathology and Intraocular Tumors
If the adjacent uvea is damaged, uveal fibrovascular tissue may enter the scleral wound, resulting in a scar with a dense adhesion between uvea and sclera. Indolent episcleral fibrosis produces a dense coat around an extrascleral foreign body such as a scleral buckle. Limbus The limbus is a complex region of corneal, scleral, and episcleral tissues. Wounds of the limbus cause swelling in the cornea and shrinking of the sclera (Fig 2-3). Healing involves episcleral ingrowth and clear corneal fibroblastic migration.
Microscopic examination reveals the different staining patterns of each core (f). J time. Protein expression is presently evaluated, for research purposes mainly, in large series of tumors and tissues included in tissue microarrays (TMAs). Tissue microarrays are paraffin blocks constructed with multiple cores of different tumors/tissues, including positive controls. Microscopic slides are prepared from these blocks and immunohistochemistry performed on a single slide for the entire group of samples (Fig 4-3).
The results may be imaged by a multicolored dot-plot histogram (Fig 4-5). The most common use of flow cytometry in clinical practice is for immunophenotyping hematopoietic proliferations. This procedure may be performed on vitreous, aqueous, or ocular adnexal tissue. In addition, multiple antibodies and cellular size can be analyzed, and the relative percentages of cells may be displayed. For example, CD4 (helper T cells), CDS (suppressor T cells), both CD4+ and CDS+, or either CD4+ or CDS+ may be displayed for a given lymphocytic infiltrate.